Variegated Leaves. 285 
1896 from some self-fertilized variegated Ocnothcra La- 
uuirckiana, although these two sorts are ordinarily con- 
stant from seed. Variegated Oenothera rubrinervis gave 
rise to 20% variegated seedlings (1892), but on a repe- 
tition of the experiment with another plant (1893) all the 
offspring were green. 
In sectorial variegation we might expect the seeds of 
the variegated sectors to give rise to more variegated 
plants than those of the green ones. The only informa- 
tion relating to this question as far as I know is due to 
HEiNSius. 1 He found a stem of Dianthus barbatns, one 
of the longitudinal halves of which was variegated, whilst 
the other was colored in the ordinary w r ay. During the 
flowering period the plant was protected from insects by 
gauze and artificially fertilized, each flower being polli- 
nated with pollen from another in the same longitudinal 
half. On the one half the capsules were white, on the 
other green ; both produced ripe seed. The seeds of the 
white fruits produced seedlings without chlorophyll but 
the seedlings from green capsules were the normal green. 
In 1888 I myself collected the green and the variegated 
fruits of a sectorial main stem of Oenothera Lamarckiana 
separately. The seeds of the former gave rise almost 
exclusively to green plants, those of the latter to a large 
proportion of variegated ones. In the summer of 1895 
I saved the fruits from a green and from a variegated 
branch of the same plant of this species, but both sets of 
seeds gave about the same very small proportion of varie- 
gated specimens, viz., 2%. 
In the summer of 1898 I conducted a more exhaustive 
research with sectorial variegation in Oenothera La- 
1 H. W. HEINSIUS in the Proceedings of the Gcnootschap ter 
bevordering der Natunr- Genees- en Heelkunde te Amsterdam. Meet- 
ing of May 7, 1898. 
