4. Life Cycle: Look for various stages in the life history; 

 encysted specimens, small individuals, large ones, etc. Also note 

 various kinds of Amoebae, some with short lobe-like pseudopodia, 

 others with longer slender ones; some large and coarsely granular, 

 others small and finely granular. 



5. Stained Specimens : In a prepared specimen, stained and 

 mounted, observe nucleus, ectosarc, endosarc, etc. Draw. 



B. PHYSIOLOGY. 



1. Movements: Is motion continuous? Regular? How is it 

 produced? Watch process of formation of a pseudopodium. What 

 part does the ectosarc play in the process ? The endosarc ? Watch 

 an active Amoeba and trace on paper its path of motion. Are there 

 permanent anterior and posterior ends ? Does there seem to be 

 any difference in surface tension between the anterior and posterior 

 ends? Are the currents in the endosarc constant? Indicate in a 

 drawing the course of the currents bv arrows. Where are the 



^J r' 



currents swiftest? Where slowest? Are cilia present on any por- 

 tion of the body ? 



2. Nutrition: If possible watch the process of taking in food 

 and of its egestion. What does the animal eat? How and where 

 does it take in food? Are food vacuoles formed? Is there a 

 definite course of circulation of food within the body? Where is 

 the food digested? How distributed? How are gaseous, liquid 

 and nitrogenous waste substances expelled from the body ." 



3. Reproduction is difficult to observe and may be omitted from 

 notes. 



4. Irritability : Are there any indications that Amoeba is sensi- 

 tive to mechanical stimuli? Is it sensitive to chemicals? To changes 

 in temperature? To electricity? To light? 



Does Amoeba show any reflex movement? Is it behavior more 

 or less varied than that of Paramecium. 



Make careful drawings and notes of all that you have observed. 



V. EUGLENA VIRIDIS, 



(A Flagellate ). 



Place a drop of water containing Euglena on a slide and after 

 covering look with the low power for green spindle-shaped organ- 

 isms which swim swiftly. Having found them study with the high 

 power and note: (i) Size. (2) Color, due to chlorophyll. The 

 anterior end is colorless. X"ear the anterior end is a red pig- 



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