ment spot, the stigma. (3) Shape, fusiform; the anterior end is 

 blunter than the posterior and bears a long flagellum which may be 

 lost in some specimens. The flagellum arises from the bottom of 

 the gullet, or mouth opening. Observe the contractile vacuole, 

 nucleus and the paramylum bodies near the center of the body. 

 What color do the paramylum bodies take when stained with iodine ? 

 What does this indicate? Is there any cell sac? 



Look for animals in the encysted condition, showing stages in 

 division. Determine by the use of Schultze's solution whether or 

 not there is cellulose in the cyst. 



Movements are of two kinds: (a) Rapid swimming movements 

 in which the flagellum is carried forwards, (b) Worm-like move- 

 ments, contractions and expansions by which the animal crawls 

 about. The latter movements are characteristic of Euglena and 

 are called "euglenoid" movements. Draw at intervals to show 

 changes in shape. 



Make drawing to show all that you have observed. 



VI. SPHAERELLA (HAEMATOCOCCUS) 



(A Flagellate Protophyte). 



A. RESTING STAGE. 



( i ) . Spread out in water some sediment containing Sphaerella, 

 put on a cover glass and look with low power for red or green 

 spheres. Having found one examine with high power and note : 

 (a). Size, variable; draw several to scale. 

 ( b ) . Form ; spheroidal. 



(c). Structure; a sac surrounding the contents which con- 

 sist of protoplasm, chromatophores, a nucleus and 

 sometimes a vacuole. 

 (d ). Color; red, green or partly one and partly the other. 



Where is the coloring matter always situated? 

 (2). Place a drop of iodine solution on the slide at the edge 

 of the cover glass, apply a bit of blotting paper at the other side, 

 thus drawing the iodine solution under the cover. What parts 

 stain ? How does it affect the nucleus and the chromatophores ? 



(3). Look for individuals in the process of division, some elon- 

 gated with transverse lines of division, others divided into two or 

 more smaller portions often lying within the sac of the parent 

 Haematococcus. Draw various stages in this division b\ fission. 



22 



