food vacuole, and from the body. SJww by a drawing where and 

 how this takes place. 



6. Excretion. The contractile vacuoles are excretory organs for 

 getting rid of water and nitrogenous waste (urea). Study and 

 sketch a vacuole in various stages of contraction and expansion. 

 Time the contractions and expansions and record the results in your 

 notes. Place Paramecia in a thick solution of Chinese ink and ob- 

 serve the extrusion of a clear drop of fluid at the moment of con- 

 traction of the contractile vacuole. 



7. Respiration. Place a number of the animals in a drop of 

 phenolpthalein ( which loses color in the presence of carbon 

 dioxide) ; also note the manner in which the animals collect at the 

 surface of a dish in which the water is very foul. What do these 

 observations teach ? Are there any organs of respiration ? 



II. REPRODUCTION. 



1. Fission. Observe and draw a Paramecium in the process of 

 division. In a stained preparation note what changes take place in 

 the nucleus and micronucleus during this process. Can you detect 

 any difference between the two daughter individuals? How r do the 

 contractile vacuoles, the buccal grooves and the gullets arise in 

 the two ? 



2. Conjugation. Study and draw living individuals in the act 

 of conjugation. What portions of the body are in contact? Is there 

 anv distinction of size or sex in the t\vo individuals? In stained 



/ 



preparations study the nuclear changes which take place during con- 

 jugation. Draw. Isolate an individual in a watch glass full of 

 water and note how many individuals are present on the next day. 



III. IRRITABILITY. 



1. Automaticity. Does the animal appear to act of its own 

 accord or only through the influence of external stimuli? 



2. Movement. How many kinds of movement does the animal 

 exhibit? What are the organs of locomotion? With a dissecting 

 microscope observe the movements of an animal in a drop of water. 

 Does it move in straight lines ? Does it keep one side uppermost ? 

 How does it alter its course ? Can it move backwards ? By means 

 of lines and arrows plot the movements of an animal during one 

 minute. By means of powdered ink observe the direction of cur- 

 rents over the body. What is the direction of the currents in the 



^ 



buccal groove ? 



3. Sensitivity. Is the animal sensitive to touch or pressure ? How 



