368 THE BEGINNINGS OF LIFE. 



Three fungus-spores with thick double walls were 

 seen. Each of these was about -g^Vo" m diameter. 

 Within one of them there were only a number of 

 granular particles (c), but within each of the other two 

 there was a large and somewhat irregular nuclear mass. 



In addition there was found the complete fungus 

 which is represented in the figure (<?), with all its 

 spores, and in a portion of one of the granular aggrega- 

 tions, a mass of about thirty spores seemed to be under- 

 going evolution, by a differentiation of mucoid material 

 through which some fine granules were disseminated. 



Experiment 4. A closed flask containing a solution 

 of neutral ammonic tartrate and neutral sodic phos- 

 phate 1 was opened on the 75th day after it had been 

 sealed 2 . 



Before the opening of the flask it was ascertained 3 

 that the vacuum had been well preserved. The reaction 

 of the fluid was still slightly acid. For a long time the 

 contents of the flask seemed to remain unaltered, though 

 for the last few weeks a very small amount of greyish 

 deposit had collected at the bottom of the vessel. 



When examined microscopically this deposit was 

 found to be principally made up of amorphous granules, 



1 In the proportion of gr. xv. of the former to gr. v. of the latter in one 

 ounce of distilled water. 



The flask having been kept during this time in a warm water-bath 

 which was constantly maintained at a temperature of 95-90 F. 



5 By the inbending of the neck of the flask when heated. It had been 

 kept with its neck immersed in the fluid, so that if this had become 

 cracked the bath fluid would have been sucked into the flask. 



