186 INJURY, RECOVERY, AND DEATH 



remained there for an hour ; 240 minutes later, when the 

 experiment was discontinued, the resistance was 111.4%. 

 The results are shown in Fig. 81. 



The effect of higher concentrations of ether was next 

 investigated. Tissue was placed in a mixture of 970 c.c. 

 sea water + 30 c.c. ether + 15 c.c. of concentrated sea 



110- 



100- 



90- 



80 



x Ether 1 % 



D ^ 2.96% 



O Sea Water. 



oC 



200 



MINUTES 400 



Fia. 81. Curve A shows the net electrical resistance of Laminaria agardhii in sea water, 

 Curve B, unbroken line, in sea water containing 1% ether (.099 M), the solution being fre- 

 quently renewed, broken line in sea water; Curve C, unbroken line in sea water containing 



2.96% ether (0.293 M), broken line, in sea water. 



water, which was added to make the conductivity of the 

 mixture equal to that of sea water. The concentration of 

 the ether was therefore 2.96% by volume (= 0.293 If). In 

 the course of 10 minutes the resistance rose to 112% ; dur- 

 ing the next 10 minutes it fell to 105.3% ; it continued to 

 fall rapidly during the next 40 minutes, reaching 89.5% at 

 the end of this period. The tissue was then placed in sea 

 water; in the next 10 minutes, the resistance fell to 87%. 

 This fall in resistance was doubtless due to the continued 

 action of the ether, which required time to diffuse out of 

 the tissue. During the next 10 minutes, there was a rise 



