ANESTHESIA 



185 



from the solution influenced the result, another experi- 

 ment was performed in which the solution was renewed 

 every 5 minutes during the first 60 minutes, and there- 

 after every 15 minutes. In this way the concentration of 

 ether was kept more nearly constant. It was then found 

 that the resistance rose as before, but did not fall during 



A Sea Water. 



B Ether 1% (= .099 



solution not renewed. 

 C " 1% solution renewed. 



100- 



904 



MINUTES 500 



Fia. 80. Curve A shows the net electrical resistance of Laminaria agardhii in sea water; B 

 in sea water containing 1% ether by volume (.099 M) from which the ether was allowed to 

 evaporate in an open dish; C in the same mixture in which the concentration of ether was 



maintained by frequent renewal. 



the first 80 minutes, and after this fell very slowly, so 

 that after 300 minutes it was still 80 ohms above that of 

 the control. At this point, the experiment was discon- 

 tinued. The results are shown in Fig. 80. 



In order to see whether the effect of the anesthetic 

 could be quickly reversed, some tissue was kept in sea 

 water containing 0.099 M ether for 50 minutes (the 

 solution being renewed every 5 minutes). During this 

 time the resistance rose to 113.7%. It was then placed 

 in sea water. At the end of 10 minutes the resistance 

 had fallen to 100%. It was then left in sea water contain- 

 ing 0.099 M ether (the solution being renewed every 15 

 minutes). The resistance promptly rose to 113.7%, and 



