io6 PROTOPLASM 



way in which they passed quite gradually into the fibro- 

 reticular protoplasm of the internal body. 



If from the observations described it has become very 

 probable that the pseudopodia even when apparently quite 

 hyaline possess a reticular structure, which has only become 

 unrecognisable for certain reasons, this supposition receives 

 further confirmation by treatment of the pseudopodia with 

 reagents. Although it was not always possible to demon- 

 strate a structure with certainty in killed and stained 

 pseudopodia, nevertheless I obtained preparations which 

 showed it most plainly. Fig. 3, Plate II. represents a 

 thick pseudopodium, richly branched towards the end, which 

 was rapidly killed with the chrom-osmium-acetic mixture 

 and then stained with Delafield's hsematoxylin. Throughout 

 almost the whole pseudopodium it appears composed of 

 longitudinally running fibres, and the structure can be dis- 

 tinctly recognised as made up of meshes, especially towards 

 the base and the extremity of the pseudopodium. In the 

 thin pseudopodial ramifications, which radiate out from the 

 main stem, I was unable to find any structure ; like the 

 main stem, however, they everywhere show a dark, some- 

 what more strongly stained border. 



Amoebae 



On various occasions" I have studied several representatives 

 of the genus Amoeba and the nearly allied Cocliliopodium. 

 Since I directed my attention principally towards the struc- 

 tural relations of the protoplasm, and had no intention of 

 studying the forms observed from all points of view, I will 

 only report upon these questions in their proper connection, 

 and will not enter into detailed descriptions. 



In the smaller Amcehcv investigated, such as A. (Dactylo- 

 sphcerium) radiosa, Ehrb., in its various forms, and A. Umax, 

 the net-like meshwork of the granular internal protoplasm 

 or endoplasm can frequently be plainly recognised even in 

 the living condition. In living Amoebse also I was often 

 able to convince myself of the existence of a radiating layer 

 round the contractile vacuole and of a similar layer round 



