A Nezv Endophytic Alga. 43 



lacerata . . . pyrenoide unico instructa. Propagatio zoogonidiis e plasmatis 

 divisione simultanea ortis et sine copulatione e membrana cellulse 

 matricalis examinantibus.' 



Chlorocystis Sarcophyci departs from the generic character in the absence 

 of any cellulose protuberance or neck-like portion. This, however, would 

 not appear to be a very important point, for Dr. Perceval Wright, to whom 

 I am indebted for very kindly examining some of my slides, informs me 

 that, when he has found Cli. CoJinii developed in the interior of a cell- 

 tissue, the cells are sometimes quite globular. (See also Kjellman, loc. cif., 



figs. 10, 17.) 



There is difficulty in explaining the division of the protoplasm into the 

 larger segments I have described above. I thought at first that, in some 

 cases at least, the protoplasm might become thus segmented before the 

 final differentiation into spores. Since, however, I have never found any 

 of the segments in question differentiated into small spores, which always 

 arise, on the other hand, by differentiation of the original homogeneous 

 protoplasm, this interpretation is not tenable. A somewhat similar 

 segmentation of the protoplasm was observed by Cohn* in the cells of 

 Chlorochytrium Lemnce and was regarded by him as a stage in the 

 formation of the spores. This observation was, however, not confirmed 

 by Klebsf, who found the spores to be formed by repeated division of the 

 protoplasm into two. 



Another possible interpretation of the segments is that they are larger 

 spores, in which case this would present a parallel with the small and large 

 spores of Chlorocystis Cohnii. Since, however, in the material I have ex- 

 amined I have found these segments, though fairly regular in size, to lack 

 uniformity of shape and in many cases the definitely rounded-off appearance 

 of the small spores, I should not be justified in offering this explanation with 

 any degree of certainty. The nature of these segments must therefore be left 

 open to be decided by the result of future investigation. An examination of 

 fresh material would probably offer an immediate solution of the difficulty. 



There remains only to be described the mode in which the endophytic 

 cells produce the malformations, and the manner of their escape from the 

 tissue. 



The malformations exhibiting considerable variations in external 

 appearance and minute structure, it will be convenient to describe them 

 at three arbitrarily chosen stages. 



There are, in the first place, those which, while presenting the usual 

 bulging of the frond, have their surfaces smooth and not decomposed. 



* Beilrdge, bd. i., heft 2. t Bot. Zeit., vol. xxxix. 



