748 HISTOLOGIC TECHNIC 



f l'li is method gives an excellent stain for the finer nuclear structure, 

 mitosis, etc. 



Muchematein (Mayer} : 



Hemateiu (Griibler's) 0.2 grm. 



Glycerin 40 c.c. 



Aluminium chloric! 0.1 grm. 



Distilled water 60 c.c. 



Mix the ingredients in the order given, rubbing the hematein with 

 the glycerin in a mortar. One or two drops of nitric acid added to the 

 final mixture will sharpen its properties as a nuclear stain. 



This dye is used as a specific stain for mucinous tissues. It is used 

 in the same manner as hematein, and stains rapidly (three to ten 

 minutes). 



Mucicarmin (Mayer) : 



Carmin 1 grm. 



Aluminium chlorid 0.5 grm. 



Distilled water 2 c.c. 



50 per cent, alcohol 100 c.c. 



Mix in the order given ; heat over a small flame till the fluid darkens 

 (two minutes) ; after twenty-four hours, filter. For use, dilute with 

 five to ten volumes of 50 per cent, alcohol. Like muchematein, mucicar- 

 min is a~ specific stain for mucus-containing cells. It also stains 

 raj) idly. 



Weigert-Pal Stain for Medullated Nerve Fibers. The tissues 

 must have been previously fixed in MiiHer's fluid or in 10 per cent, 

 formalin, washed in water, hardened in alcohol, and sectioned. 



I. Stain: 



Hematoxylin 1 grm. 



Absolute alcohol 10 c.c. 



Distilled water 90 c.c. 



Saturated aqueous solution of lithium carbonate 



(1 to 80) 1 c.c. 



II. Differentiating Solution: 



Potassium permanganate 0.25 grm. 



Distilled water , 100 c.c. 



