264 PREPARATION OF SECTIONS 



dish containing one part of hydrogen peroxide to twenty parts 

 of 60 per cent, alcohol. 



Staining the Sections. The finest results in staining are 

 obtained with Flemming's triple stain, safranin, gentian violet 

 and orange G, made by Griibler. Prepare the stains separately 

 as follows: Make a saturated solution of safranin in 95 per 

 cent, alcohol and dilute it with an equal amount of distilled 

 water. Make a saturated solution of gentian violet in distilled 

 water, to be used without dilution. Make a saturated solu- 

 tion of orange G in distilled water and dilute it with five times 

 its bulk of distilled water. Put the safranin and gentian 

 violet into Stender dishes or tightly covered tumblers, and the 

 orange G into a drop bottle. In addition to the stains have 

 conveniently at hand: 



A drop bottle containing absolute alcohol. 



A drop bottle containing clove oil. 



A Stender dish or tumbler of xylene. 



A Stender dish or tumbler of 95 per cent, alcohol acidulated 

 with a drop of concentrated hydrochloric acid. 



Proceed with the staining as follows: 



1. Stand the slide on end in the safranin for a few hours 

 or over night. 



2. Remove the slide from the safranin, drain it, rinse it 

 quickly in water, and set it on end in the dish of acidulated 

 alcohol until the safranin stops coming off in clouds and the 

 sections seem almost or quite decolorized. 



3. Rinse the slide quickly in water and set it on end in the 

 dish of gentian violet for ten minutes. 



4. Remove the slide from the gentian violet; rinse it in 

 water, hold it horizontally, and flood the sections with orange 

 G from a drop bottle for four seconds. 



5. Rinse off the orange G in water, drain the slide, and 

 while .holding it slightly slanting downward thoroughly dehy- 

 drate the sections by having absolute alcohol flow over them 

 from the drop bottle. 



6. Set the slide horizontally and flood the sections with 



