294 Methods in Plant Histology 



PTERIDOPHYTES 



LYCOPODIALES 



50. Lycopodium lucididum. Transverse section of stem. Freehand sections. 

 Safranin and Delafield's haematoxylin. 



51. Lycopodium inundatum. Paraffin. Longitudinal sections of strobilus. 



52. Selaginella. Paraffin. Longitudinal sections of rather mature strobili. 

 Cyanin and erythrosin, or safranin, gentian-violet, orange. 



53. Isoetes echinospora. Transverse section of stem. Paraffin. Safranin 

 and Delafield's haematoxylin. 



54. Isoetes echinospora. Paraffin. Longitudinal sections of microsporangia 

 and megasporangia. Safranin, gentian- violet, orange. 



EQUISETALES 



55. Equisetum arvense. Prothallia in Venetian turpentine. Stem-tips in 

 paraffin. Transverse section of stem freehand or in celloidin. 



OPHIOGLOSSALES 



56. Botrychium virginianum. Paraffin. Stain rhizome, stipes, and root 

 in safranin and Delafield's haematoxylin. Stain sporangia in iron- 



haematoxylin. 



FILICALES 



57. Protostele. Use Gleichenia. Cut freehand and stain in safranin and 

 anilin blue. 



58. Solenostele (amphiphloic siphonostele). Use Adiantum. 



59. Ectophloic siphonostele. Use Osmunda cinnamomea. 



60. Polystele. Use Pteris aquilina or any species of Polypodium. 



61. Sporangia. For development, use Pteris, Aspidium, Cyrtomium, or 

 try any available species. For mitosis, Osmunda is exceptionally good. 



62. Antheridia and archegonia. Mount whole in Venetian turpentine. 

 Magdala red and anilin blue. Sections should be 5 to 10 /x thick. 

 Stain in iron-haematoxylin and orange. 



63. Embryo. Pteris and Adiantum are good. Cut longitudinal vertical 

 sections 10 /x thick. 



SPERMATOPHYTES 



GYMNOSPERMS 

 CYCADALES 



64. Zamia. Freehand sections of stem. Safranin and light green. Trans- 

 verse sections of microsporophyll, 5 or 10 /x. Longitudinal sections of 

 entire ovule, 10 to 15 /x; stain in safranin, gentian- violet, orange. 

 Longitudinal sections of nucellus with pollen tubes, 10 /x. Iron- 

 haematoxylin and orange. 



