CHAPTER XXVIII 

 A CLASS LIST OF PREPARATIONS 



Where a regular course in histology is conducted, it is a good plan 

 to give each student at the outset a complete list of the preparations 

 which he is expected to make. In a three months' course a fairly 

 representative collection of preparations can be made. The avail- 

 ability of material determines what a list shall be. Besides gaining 

 an introduction to the use of the microscope and its accessories, a class 

 meeting ten hours a week for twelve weeks should be able to do as 

 much work as is outlined below. 



In making the mounts the order indicated in the list should not 

 be followed. Begin with temporary mounts, and then study, in 

 succession, freehand sections (the glycerin method), the Venetian 

 turpentine method, the paraffin method (the celloidin method), and 

 special methods. A large proportion of the time should be devoted 

 to the paraffin method. 



It is neither possible nor desirable that each student should in 

 every case go through all the processes from collecting material to 

 labeling. Some of the material may be in 85 per cent alcohol, some 

 in formalin, some in glycerin, some in Venetian turpentine, and some 

 in paraffin. One student may imbed in paraffin enough of the 

 Anemone for the whole class; another may imbed the Lilium stamens; 

 and by such a division of labor a great variety of preparations may 

 be secured without a corresponding demand upon the time of the 



individual. 



LIST OF PREPARATIONS 



THALLOPHYTES 



SCHIZOPHYTES 

 MYXOMYCETES 



1. Trichia varia. Paraffin sections, 5 /x. Safranin, gentian-violet, orange. 



SCHIZOMYCETES 



2. Bacteria. Coccus, Bacillus, and Spirillum forms. Stain on cover-glass 

 or slide. 



3. Bacillus anthracis. In liver of mouse. Paraffin sections, 5 /x. Stain 

 in gentian-violet, Gram's method. 



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