292 Methods in Plant Histology 



19. Spirogyra. Stain in Magdala red and anilin blue, and mount in Venetian 

 turpentine. 



20. Vaucheria. Stain in Magdala red and anilin blue, and mount in Venetian 

 turpentine. 



21. Char a. Cut paraffin sections of the apical cell, oogonia, and antheridia. 



PHAEOPHYCEAE 



22. Ectocarpus. Stain some in iron-haematoxylin and some in Magdala 

 red and anilin blue. Mount both together in Venetian turpentine. 



23. Cutleria. Sections of oogonia, antheridia, and sporangia. Cut 10 ^ 

 thick and stain in iron-haematoxylin with about 7 minutes in safranin. 



24. Fucus vesiculosus. Antheridial conceptacle with paraphyses and 

 antheridia; oogonial conceptacle with oogonia. Cut 10 /a thick and 

 stain in iron-haematoxylin with about 5 minutes in safranin. 



RHODOPHYCEAE 



25. Nemalion. Stain some in iron-haematoxylin and some in eosin. Each 

 preparation should show trichogyne, carpogonium, and cystocarp. You 

 cannot mount it in Venetian turpentine: use glycerin or glycerin jelly. 



26. Polysiphonia. Stain in iron-haematoxylin or Magdala red and anilin 

 blue. Mount whole in Venetian turpentine. Each mount should 

 show tetraspores, antheridia, and cystocarps. If material is in paraffin, 

 cut sections about 7 /x thick. 



FUNGI 

 PHYCOMYCETES 



27. Mucor stolonifer. Stain young sporangia in eosin, dilute Delafield's 

 haematoxylin, or in Magdala red and anilin blue. Zygosporic material 

 may be mounted without staining or after a very light staining in dilute 

 Delafield's haematoxylin. Eosin is also good. Mount in Venetian 

 turpentine. 



28. Saprolegnia. Stain in Magdala red and anilin blue. Mount in Venetian 

 turpentine. Each mount should show sporangia and oogonia. 



29. Albugo (Cystopus) candidus. Select white blisters which have not yet 

 broken open. Paraffin, 5 /x. Iron-haematoxylin and orange. 



30. Albugo bliti on Amarantus retroflexus. Cut out small portions of leaves 

 in which the oogonia can be seen in abundance. Paraffin, 5 /x. 



ASCOMYCETES 



31. Peziza. Paraffin sections of young apothecia, 5 /x or less; sections of 

 older apothecia, 10 or 15 /x. Safranin, gentian-violet, orange. 



32. Aspergillus, Eurotium. Stain in eosin and mount in glycerin, or stain 

 in Magdala red and anilin blue, and mount in Venetian turpentine. 



33. Penicillium. Treat like Aspergillus. 



