A Class List of Preparations 293 



34. Erysiphe commune on Polygonum aviculare. Strip the fungus from the 

 leaf. Paraffin, 5 /x or less. Safranin, gentian- violet, orange. 



35. Uncinula necator on Ampelopsis quinquefolia. Stain in Magdala red 

 and anilin blue. Mount whole in Venetian turpentine and break the 

 perithecia under the cover. 



36. Xylaria. Paraffin sections of younger stages. DelafiekTs haematoxylin 

 and erythrosin. Be sure that some section in each mount shows the 



opening of a perithecium. 



LICHENS 



37. Physcia stellaris. Cut in paraffin, 5 /x. Stain in cyanin and erythrosin. 



BASIDIOMYCETES 



38. Puccinia graminis. Aecidium stage on barberry leaf. Uredospore 

 and teleutospore stage on oats. Cut 3 /x and stain in iron-haematoxylin. 



39. Coprinus micaceus. Paraffin. Transverse sections of gills showing 

 trama, paraphyses, basidia, and spores. To show the basidium with 

 four spores, the sections should be 15 /x thick. For development of the 

 spores, cut 5 /x or less. Safranin, gentian-violet, orange. Boletus, 

 Hydnum, and Polyporus are treated in the same manner. 



BRYOPHYTES 



HEPATICAE 



40. Riccia natans. Paraffin, 10 or 15 tt. Delafield's haematoxylin. Arche- 

 gonia, antheridia, and sporophytes imbedded in the gametophyte. 



41. Marchantia polymorpha. Paraffin, 5 or 10 /*. Archegonia, antheridia, 

 and sporophytes. 



42. Anthoceros laevis. Paraffin, 5 or 10 /x. Longitudinal and transverse 

 sections of sporophyte. Safranin, gentian-violet, orange. 



43. Pellia epiphylla. Paraffin, 5 or 10 tt. Longitudinal sections of sporo- 

 phyte attached to gametophyte. Safranin, gentian-violet, orange. 



44. Porella platyphyllum. Paraffin, 10 /x. Delafield's haematoxylin. Arche- 

 gonia, antheridia, sporophyte, and apical cell. 



MUSCI 



45. Sphagnum. Leaf buds. Cut 5 /x and stain in safranin and anilin blue. 



46. Sphagnum. Capsule. Paraffin. Delafield's haematoxylin and eryth- 

 rosin. 



47. Funaria hygrometrica. Paraffin. Longitudinal and transverse sections 

 of young capsules. Delafield's haematoxylin. 



48. Funaria hygrometrica or any favorable form. Protonema. Place the 

 well-cleaned material directly into 10 per cent glycerin and allow it to 

 concentrate. Mount in glycerin or glycerin jelly. 



49. Bryum proliferum. Paraffin. Antheridia, 10 /x; archegonia, 15 to 

 20 /x; capsule, 10 /x. 



