298 Methods in Plant Histology 



Chromo-Acetic Solution (for delicate structures) .- 



Chromic acid 1 g. 



Glacial acetic acid 3 c.c. 



Water 300 c.c. 



The addition of 10 drops of osmic acid to 50 c.c. of any of the 

 chromo-acetic solutions is often an advantage. 



Chromo-Acetic Solution (for marine algae). - 



Chromic acid 1 . g. 



Glacial acetic acid . 4 c.c. 



Sea-water 400. c.c. 



Material must be washed in sea-water. 



Flemming's Fluid (weaker solution) .- 



f 1 per cent chromic acid (in water) 25 c.c. 



A 1 per cent glacial acetic acid (in water) .... 10 c.c. 

 I Water 55 c.c. 



B. 1 per cent osmic acid (in water) 10 c.c. 



Keep the mixture A made up, and add B as the reagent is needed 

 for use, since it does not keep well. 



Flemming's Fluid (stronger solution). - 



1 per cent chromic acid 45 c.c. 



2 per cent osmic acid 12 c.c. 



Glacial acetic acid 3 c.c. 



Merkel's Fluid. 



1.4 per cent solution of chromic acid 25 c.c. 



1.4 per cent solution of platinic chloride 25 c.c. 



Benda's Fluid.- 



1 per cent chromic acid 16 c.c. 



2 per cent osmic acid 4 c.c. 



Glacial acetic acid 2 drops 



Hermann's Fluid.- 



1 per cent platinic chloride 15 parts 



Glacial acetic acid 1 part 



2 per cent osmic acid 4 or 2 parts 



