296 Methods in Plant Histology 



MONOCOTYLS 



83. Clintonia. Transverse section of stem to show siphonostele. Freehand. 

 Safranin and anilin blue. 



84. Acorus calamus. Transverse sections of rhizome, freehand or paraffin, 

 to show amphivasal bundles. 



85. Zea Mays. Transverse section of stem to show scattered bundles; also 

 good for companion cells. Freehand. Safranin and anilin blue. 



86. Tradescantia virginica. Longitudinal sections of root-tip. Paraffin, 

 5 and 10 /a. Stain for mitosis. 



87. Smilax herbacea. Transverse section of adult root. Freehand. Shows 

 exarch, radial structure, and a highly developed endodermis. Safranin 

 and Delafield's haematoxylin. 



88. Lilium. Transverse section of leaf. Freehand. Transverse sections 

 of ovaries in various stages from megaspore mother cell to fertilizations; 

 transverse sections of anthers to show microspore mother cells and reduc- 

 tion of chromosomes; also later stages with nearly mature pollen. 

 Paraffin 5 to 10 /A. 



89. Iris. Section of } r oung seeds to show embryo with cotyledon and 

 stem-tip. 



90. Sagittaria. Longitudinal sections of ovulate flowers of various stages 

 to show development of the embryo and endosperm. 



91. Zea Mays. Longitudinal and transverse sections of embryo (sweet corn, 

 roasting-ear condition) to show structure of root and beginning of pro- 

 toxylem. 



