Formulae for Reagents 299 



Picric Acid.- 



Picric acid 1 g. 



Water or 70 per cent alcohol 100 c.c. 



Bouin's Fluid.- 



Commercial formalin 25 c.c. 



Picric acid (saturated solution in water) 75 c.c. 



Glacial acetic acid 5 c.c. 



Corrosive Sublimate and Acetic Acid.- 



Corrosive sublimate 3 g. 



Glacial acetic acid 3 c.c. 



70 per cent alcohol (or water) 100 c.c. 



Bensley's Formula (for mitochondria) .- 



2 1 per cent corrosive sublimate in water 4 parts 



2 per cent osmic acid 1 part 



Corrosive Sublimate, Acetic Acid, and Picric Acid.- 



Corrosive sublimate 5 g. 



Glacial acetic acid 5 c.c. 



Picric acid 1 g. 



50 per cent alcohol 100 c.c. 



Corrosive Sublimate and Picric Acid (Jeffrey's formula). - 

 Corrosive sublimate, saturated solution in 30 



per cent alcohol 3 parts 



Picric acid, saturated solution in 30 per cent 



alcohol 1 part 



Gilson's Fluid.- 



95 per cent alcohol 42 c.c. 



Water 60 c.c. 



Glacial acetic acid 18 c.c. 



Concentrated nitric acid 2 c.c. 



Corrosive sublimate (saturated solution in 



water) 11 c.c. 



Bensley's Formula (for canal system) .- 



1 . Bichromate of potash 2| g. 



2. Corrosive sublimate 5 g. 



3. Water 90 c.c. 



4. Formalin (neutral) 10 c.c. 



Make a solution of 1, 2, 3, and then add the neutral formalin. 



