302 Methods in Plant Histology 



Picro-Carmine.- 



Picro-carmine (picro-carminate of ammonia) . . 1 g. 

 Water 100 c.c. 



Eosin.- 



Eosin 1 g. 



Water, or 70 per cent alcohol 100 c.c. 



General Formula for Anilins. Make a 3 per cent solution of 

 anilin oil in distilled water; shake well and frequently for a day; 

 add enough alcohol to make the whole mixture about 20 per cent 

 alcohol; add 1 g. of cyanin, erythrosin, safranin, gentian-violet, etc., 

 to each 100 c.c. of this solution. 



Cyanin. This general formula is not at all successful with 

 Gruber's cyanin, but gives satisfactory results with an immensely 

 cheaper cyanin, sold by H. A. Metz & Co., 122 Hudson Street, New 

 York. 



Anilin Blue.- 



Anilin blue 1 g. 



85 or 90 per cent alcohol 100 c.c. 



For staining before mounting in Venetian turpentine, this stain 

 should be made up in strong alcohol, even if the dry stain is intended 

 for aqueous solution. 



Iodine Green.- 



lodine green 1 g. 



70 per cent alcohol 100 c.c. 



Methyl Green.- 



Methyl green 1 g. 



Glacial acetic acid 1 c.c. 



Water 100 c.c. 



If the preparation is to be mounted in balsam, a slight trace of 

 acetic acid and also a trace of methyl green should be added to the 

 absolute alcohol used for dehydrating. 



1 For staining vascular bundles, the acid may be omitted, even from 

 the formula. 



