Formulae for Reagents 303 



Light Green. 



Light green 1 g. 



Clove oil 100 c.c. 



or 



Light green , 1 g. 



Clove oil 75 c.c. 



Absolute alcohol 25 c.c. 



Fuchsin.- 



Fuchsin 1 g. 



95 per cent alcohol 100 c.c. 



Water 100 c.c. 



Acid Fuchsin.- 



Acid fuchsin 1 g. 



Water 100 c.c. 



Use this formula when staining woody tissues in methyl green 

 and acid fuchsin. 



Ziehl's Carbol Fuchsin.- 



Fuchsin 1 g. 



Carbolic-acid crystals 5 g. 



95 per cent alcohol 10 c.c. 



Water 100 c.c. 



Fuchsin and Iodine Green Mixtures.- -Two solutions are kept 

 separate, since they do not retain their efficiency long after they are 

 mixed. 



f Fuchsin (acid) . 1 g. 



\ Distilled water 50. c.c. 



j Iodine green . 1 g. 



\ Distilled water 50.0 c.c. 



f Absolute alcohol 100. c.c. 



C \ Glacial acetic acid 1.0 c.c. 



( Iodine . 1 g. 



Stain in equal parts of A and B. Transfer from the stain directly 

 to solution C, and from C to xylol. 



