304 Methods in Plant Histology 



Another Formula.- 



J Acid f uchsin . 5 g. 



A \ Water 100.0 c.c. 



f Iodine green . 5 g. 



\ Water 100. c.c. 



Mix a pipette full of A with a pipette full of B ; stain 2 to 8 min- 

 utes; dehydrate rapidly and mount in balsam. 



Magdala Red.- 



Magdala red 1 g. 



85 or 90 per cent alcohol 100 c.c. 



Use this formula when staining in Magdala red and anilin blue, 

 before mounting in Venetian turpentine. 



Safranin.- 



Safranin 1 g. 



95 per cent alcohol 50 c.c. 



Water 50 c.c. 



Safranin (another formula) .- 



Dissolve 1 g. of alcohol-soluble safranin in 100 c.c. of absolute alcohol; 

 dissolve 1 g. of water-soluble safranin in 100 c.c. of distilled water. Mix 

 equal parts of the two solutions. 



Gentian- Violet.- 



Gentian- violet 1 g. 



95 per cent alcohol ' 20 c.c. 



Water 80 c.c. 



Anilin oil 3 c.c. 



A 1 per cent solution in water keeps better. 



A 1 per cent solution in clove oil is worth a thorough trial. 



Pyoktanin.--This is sold by E. Merck, in Darmstadt, Germany. 

 Dissolve 1 g. of pyoktanin in 30 c.c. of water. 



Orange G.- 

 Orange G 1 g. 



Water 100 c.c. 



For most purposes a 1 per cent solution in clove oil is preferable. 



