PREFACE TO THE SECOND EDITION 



It is gratifying to the author to learn that the kindly reception 

 accorded to Methods in Plant Histology has exhausted the edition. 

 Since the first edition appeared, a little more than four years ago, 

 laboratory methods have been greatly improved, and systematic 

 experiments have made it possible to give much more definite 

 directions for making preparations. 



In the present edition much more attention has been given to 

 collecting material. Professor Klebs's methods for securing various 

 reproductive phases in the Algae and Fungi have been outlined in 

 a practical way. Methods for growing other laboratory material 

 are more complete than in the earlier edition. 



The paraffin method has been much improved, and the glycerin 

 method has been almost entirely replaced by the Venetian turpentine 

 method, to which a whole chapter is devoted. Other new chapters 

 deal with michrochemical tests, freehand sections, special methods, 

 and the use of the microscope. 



The author is deeply indebted to his colleague, Dr. W. J. G. 

 Land, for numerous suggestions and improvements in methods. 



Corrections and suggestions will be heartily appreciated. 



CHARLES J. CHAMBERLAIN 

 CHICAGO 

 July 1, 1905 



vn 



