CONTENTS 

 PART I 



PAGE 



INTRODUCTION 3 



CHAPTER I. APPARATUS . 5 



CHAPTER II. REAGENTS . 17 



Killing and Fixing Agents 17 



Dehydrating Agents .... 31 



Formulae for Alcohols 33 



Clearing Agents . . 34 



Miscellaneous Reagents 37 



CHAPTER III. STAINS AND STAINING . 38 



The Haematoxylins 40 



The Carmines 49 



The Anilins .... 50 



Combination Stains 59 



CHAPTER IV. GENERAL REMARKS ON STAINING 65 



Selection of a Stain ... 65 



Theories of Staining ... 66 



Practical Hints on Staining ... 69 



CHAPTER V. TEMPORARY MOUNTS AND MICROCHEMICAL TESTS 72 

 CHAPTER VI. FREEHAND SECTIONS .... .80 

 CHAPTER VII. THE GLYCERIN METHOD ... . .92 



CHAPTER VIII. THE VENETIAN TURPENTINE METHOD ... 97 



CHAPTER IX. THE PARAFFIN METHOD . .... 102 



Killing and Fixing . . 102 



Washing . . 104 



Hardening and Dehydrating 105 



Clearing ... . . . 106 



The Transfer from Clearing Agent to Paraffin . . . 107 



The Paraffin Bath . . 108 



Imbedding . 109 



Cutting . ... . Ill 



Fixing Sections to the Slide ... 113 

 Removal of the Paraffin . . . . .115 



ix 



