PREFACE TO THE THIRD EDITION 



The continued appreciation accorded to Methods in Plant His- 

 tology has exhausted the second edition. Since that edition appeared, 

 methods have become more and more exact, so that the present 

 volume is practically a new book. The general arrangement of the 

 subject-matter, and directions for collecting material and for secur- 

 ing reproductive phases in the Algae and Fungi have been retained, 

 and a chapter on " Photomicrographs and Lantern Slides" (chap, xii) 

 has been added. 



Great improvements have been made in the paraffin method, so 

 that sections are easily cut which were impossible ten years ago, 

 while ten years of added experience with the Venetian turpentine 

 method have made it possible to describe it so definitely that even 

 the beginner should find no serious difficulty. 



The author is deeply indebted to his colleague, Dr. W. J. G. 

 Land, for numerous suggestions and improvements covering the 

 whole field of microtechnic. He is also greatly indebted to Dr. S. 

 Yamanouchi for many improvements in the methods applicable to 

 Algae and mitotic figures. 



Corrections and suggestions will be heartily appreciated. 



CHARLES J. CHAMBERLAIN 

 CHICAGO 



May, 1915 



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