ANNULATA. 



239 



Laboratory Work. Worms should be dissected at once after be- 

 ing killed by ether or in alcohol, before the circulation has ceased ; 

 and transverse sections made to observe the relation of the appendages 

 to the body-walls, and of the different systems within the body-walls. 

 The worms should also be hardened in alcohol, and thin sections 

 stained with carmine be made for histological study. A portion of the 

 worm can be put in paraffine and sliced by hand with the razor or by 

 the microtome. 



Amphitrite oruat", 



