LOLIGO PEALEII. 143 



ejaculatory apparatus, and the cement body, which lies between 

 them. 



4. The sperm mass may be seen to consist of a closely wound 

 thread. It is actually covered by a very thin inner tunic which 

 is separated from the outer tunic by a space filled with liquid. 



5. The cement body is attached to the sperm mass by a 

 narrow core and is covered by a continuation of the inner 

 tunic. It is flask shaped, with the narrow end pointing orally. 



6. The neck of the cement body is covered by parts of the 

 ejaculatory apparatus and the bulge of the body is joined and 

 possibly covered by the outer membrane, which with the inner 

 tunic forms the outer covering of the ejaculatory apparatus that 

 appears as a double membrane. Inside the outer membrane is 

 the rather thick middle membrane, the aboral end of which 

 encloses the neck of the cement body and ends against the 

 bulge of the cement body, and the oral end of which is thrown 

 into bends and loops and is finally attached to the cap end of the 

 outer case. Inside the middle membrane is the thin inner 

 membrane and the spiral filament which enclose a narrow lumen. 



7. In ejaculating the ejaculatory apparatus turns wrong 

 side out and the cement body and sperm mass are crowded 

 down the tube thus formed by the elastic force of the outer 

 tunic and the elastic and osmotic action of the middle tunic. 



The sperm mass is forced into a sac composed of the in- 

 verted inner tunic and outer membrane, which remain at- 

 tached to the bulge of the cement body, the cement body is 

 ruptured and the cement spread over the closed end of this sac. 

 The reservoir is now ready to stick in position. 



Studying the method of ejaculation is time consuming. 

 Fresh specimens placed in about one-fourth saturated solution 

 of magnesium chlorid for ten minutes or more will be slowed 

 in action so the process can be followed more readily. Remove 

 a specimen from this solution to sea-water, grasp the cap thread 

 with forceps, and shake the spermatophore. This should start 

 ejaculation. Ejaculation can be stopped promptly by squirting 

 full strength formaldehyd on it. 



