Appendix. 1 5 3 



After dehydrating, the object is cleared, i.e., an oil, which 

 will mix with alcohol on the one hand and with Canada bal- 

 sam on the other, is allowed to take the place of the abso- 

 lute alcohol. This change for all small objects may be made 

 by lifting the object from the alcohol and, after draining off 

 the excess of alcohol, placing the specimen for a few minutes 

 in oil of clove, or oil of cedar, or turpentine, or organum oil. 

 After remaining in the oil for a few moments, the object will 

 become quite transparent. It is now ready to be placed on 

 the slide and, after the oil is drained off, covered with a drop 

 of Canada balsam. 



A clean cover-glass is now warmed for a moment over the 

 spirit-lamp and allowed to gently rest on the balsam and 

 object. If there is an excess of balsam, it may be removed 

 with a cloth dampened with turpentine. If there is not a 

 sufficient quantity of balsam, a drop may be placed on the 

 slide near the edge of the cover, when it will run under by 

 capillary attraction. 



If the object is rather thick and does not well support 

 the cover-glass, the latter may be supported with rubber 

 or glass rings made for the purpose, or with small pieces 

 of a broken slide. " Wax feet " have been recommended by 

 Kukenthal. 1 



I. 



METHODS OF IMBEDDING AND SECTION 



CUTTING. 



THOUGH sections of certain tissues may be cut without 

 previous preparation, the best results are to be obtained by 

 the adoption of one of the following methods : 



1 To three parts of wax ^e added one part of vaseline and one part 

 of Canada balsam. 



