\\VMAN REED GREEN. 



\periment 2. The following experiment was designed to 

 >how in particular the normal ratio of males to females, and 

 their sequence, though it furnishes data bearing upon several 

 other points. It was begun on June 23 by the isolation of a 

 single female from a laboratory culture. She produced the 

 following broods on the dates indicated' 



( )n June 26 a brood of 43, 27 being female, 16 not living. 



On June 28 a brood of 49 females. 



On July 3 a brood of 75 females. 



On July 7 a brood of 7 males. 



On July 10 a brood of 6 females. 



On July ii the isolated female died. 



Of the brood produced on June 28, 45 which lived were saved 

 for experiment. These were isolated and the sex of all of their 

 offspring determined, and in several instances the kind of females 

 i.e., whether sexual or asexual. The members of this brood are 

 numbered. The date of the first broods of each of the first 17 

 was July 4, of all of the remainder it was July 5. The date of 

 the death of each female is given at the end of her series of broods. 

 The date of the last brood is practically always not more than 

 one day before the death of the female. The second brood ot 

 female number I is a mixed brood and they are so indicated 

 throughout. 



1. 9 females, 5 l\-maU> and i) males, 9 females, an undeter- 

 mined brood, July 15. 



2. 9 females, a mixed brood of about 20 containing many 

 dead, 6 females, July 12. 



3. 4 females, 3 males and 6 undetermined, 11 males, 4 fe- 

 males, July 13. 



4. 4 females, 18 males and 2 females, 6 females, i female, 

 July 13. 



5. 3 females, 1 1 males and 8 females, 16 females, July 9. 



6. 9 males, 3 undetermined, 6 females, 22 females, July 10. 



7. 7 females, 7 females and S undetermined, 6 females, 5 

 females, an undetermined brood. July 14. 



8. 6 females, 3 females and 10 undetermined, 10 males, 

 July 7, 14. 



9. S females, 2 males, 4 males, July 9. 



