INFLUENCE OF GLANDULAR KX 1 K \< IN. 



\Vild Culture. 



TABLE II. 



AVERAGE RATE OF PULSATION FOR EACH GROUP. 



Anterior Vacuole. Posterior Vacuole. 



Pure line A 11.2 Pure line A 10.7 



Pure line B 15.1 



Wild Culture. . . 12.6 



Pure line B 14.2 



Wild Culture. . . n.6 



the laboratory, and (4) there is an average rate for all Paramecia 

 kept under the same physiological conditions. In this case the 

 average for the anterior vacuole is 12.9 and for the posterior 

 vacuole 12.1. These figures are remarkably close to the normal 

 average set by Ehrmann (8) which was 12. 



In the experiments with glandular extracts individuals from 

 various wild cultures were used. Since the pulsations do not 

 seem to be subject to specific pure line characteristics it was 

 thought wiser to work with individuals not kept for any length 

 of t me in the artificial environment of distilled water and malted 

 milk. It has already been shown that Paramecia taken from a 

 wild culture in spr'ng water, and placed on depression slides with 

 2 drops of distilled water and I drop of I per cent, malted milk 

 solution daily show after three and four days of this treatment 

 in a moist chamber at room temperature a perfectly normal rate 

 of pulsation. The following glandular preparations of Parke, 

 Davis & Co. were used adrenalin in crystal form, pituitary sub- 

 stance made from the desiccated anterior lobe of the pituitary 

 body, and pineal gland tablets prepared from the desiccated 



