\\VMAX REED GREEN. 



\ugust 24. Eight more hatched on August 21,7 more on 

 August 25, 10 on August 26, 6 on August 30, 4 on September ; v 

 2 September 6, I September 20, and at more or less irregular 

 intervals for several months thereafter others continued to 

 appear, until nearly six dozen had been secured. 



Two other instances deserve mention. Three days before 

 setting up this experiment I had placed a number of eggs, pro- 

 duced from May I, to June 26, in a jar in a similar manner and 

 they remained there until December 12, when they suddenly 

 began to hatch, but only a few appeared. I can suggest no ex- 

 planation as to why they did not all hatch, or win- the few that 

 did should not have done so earlier as did most of those men- 

 tioned above. On August 9 some fresh eggs were placed on ice 

 and left 36 hours and then placed under water in the manner 

 described above. On December 27 two hatched. No more 

 appeared although the experiment was left standing until June i, 

 1917, i.e., about 11 months. 



Mortality among these stem mothers was found to be about 

 the same as with other females. I succeeded in obtaining an 

 average of a little over six broods from each of 24 of these stem 

 mothers and have given their history in the interesting experi- 

 ment (number 3) already described. 



It should be stated that there were numerous other eggs in 

 the culture from which the majority of the stem mothers was 

 secured, which did not hatch. Some of the water from this 

 culture was removed and other newly produced eggs placed in 

 it. This experiment was left standing from August 30, 1916, 

 until May 5, 191?- Since none of these developed we must in- 

 fer that the kind of water in the batters- jar containing tin- 

 developing eggs, was not the responsible- factor, though I have 

 not the remotest idea what it may have been. 



VI. < )USERVATIONS ON PAIRING. 



A temale from a culture containing many ephippial females 

 was isolated on July 19. By July 27 she had produced two 

 h male broods, one ot 14 and another of 10. I placed the older 

 brood with males. On August 1st I found that 14 ephippia had 



In i'n produced, though lo of them \\ere empty, and all but one of 



