\\V.M.\.\ KKKD (;KEEN. 



offspring with which to test this question. I selected 12 of the 

 xual females of broods 12 and 13. Each of these 12, after 

 having produced one empty ephippium at the age of 11 days, 

 became asexual. Two of the 12 died. In two other cases the 

 first brood died too early for identification, but the first deter- 

 minable broods of the 10 that lived were as follows: 



i. 6 females, i sexual, 3 asexual, and 2 undetermined. 

 7 2 " 3 " " 2 



2 "2 



3 



" 2 " 



6 



5 



" 2 '* 



II 



Thus in these first broods of the 10 sexual mothers only about 

 22 per cent, of the females are sexual, and about 12 per cent, of 

 the offspring is male. All of the subsequent broods of these 

 lo mothers were also saved and identified. It was found that 

 the percentage of males was slightly over 8 per cent., a decrease 

 from that shown in the broods immediately following the ephip- 

 pia. This can be of no special significance how'ever since the 

 percentage in that case is very much lower than one usually 

 finds even in the offspring of asexual mothers, as noted in other 

 experiments. As we have already seen, somewhat over 40 per 

 cent, of the offspring, totaling over 1,700, of the 45 females con- 

 cerned in isolation experiment number 2, is male. About 33 

 per cent, of the broods of these 10 sexual mothers are mixed as 

 to sexuality, which is about normal. It will be recalled also 

 that the percentage of males and sexual females in the sexual 

 line of offspring from the stem mothers, (see isolation experi- 

 ment 3) is actually slightly less than in the asexual line. It 

 thus seems certain that the production of ephippial eggs at the 

 beginning of the reproductive period, whether they are fertilized 

 or not, has no influence on the subsequent offspring. 



3. Is the age of tin- mother correlated in any way with the 

 kind of offspring? 



The twenty-fourth female in isolation experiment 2, gave as 



