68 MARY J. ROSS. 



2436 hrs. in Delafield's hematoxylin, Del. hem., I part, -)- dis- 

 tilled water, 4 parts, then washed in distilled water 1012 hrs., 

 or until the water is no longer colored by the hematoxylin. 

 They were then stained in toto with eosin, sat. sol. in 95 per cent, 

 alcohol, washing out with absolute alcohol.) 



For the earlier stages, on account of the great amount of yolk 

 present, double imbedding with collodion and paraffin was em- 

 ployed. Thinner sections could be obtained in this way, with 

 less loss of yolk material. In thickness the sections varied from 

 two to ten micra. 



The material used in the study of the stomach of the pig was 

 placed at my disposal by the Department of Histology and Em- 

 bryology. It is a great pleasure to express my appreciation to 

 the members of this Department for the interest shown during 

 the investigation. 



This material was fixed in five per cent, formalin, mercuric 

 chlorid, alcohol, picric alcohol and Zenker's fluid. In spite of 

 the tendency of the epithelium to shrink away from the mucosa 

 the formalin material proved the best for study. Staining in toto 

 with alcoholic eosin, restaining with hematoxylin and eosin, or 

 staining in toto with Delafield's hematoxylin and alcoholic eosin 

 was employed with good success. Other stains as mentioned 

 above for DcsniogiiatJnts and Amblystoma were also used. 



All figures are drawn with an Abbe camera lucida. Unless 

 otherwise stated drawings are made with Leitz lenses and the or- 

 dinary tube length. Objective 1/16, oil immersion. 



ORIGIN OF THE GASTRIC GLANDS. 

 Desmognathus. 



Notwithstanding the general tendency of to-day to explain the 

 development of the so-called higher forms by comparison with 

 the simpler or lower, nothing has been done, as yet, along this 

 line, for the gastric glands. The embryologies are singularly 

 free from references as to the origin of glands, save in the higher 

 forms, and even here there is a dearth of helpful literature. 



To review, briefly, the facts, as known in the Batrachian forms 

 so far investigated, will aid to simplify the presentation of this 



