38 J. w. SCOTT. 



Experiment 2. The object of the experiment was to test the 

 effect of a more violent method of transferring. The eggs were 

 taken up in a pipette and squirted with moderate pressure into 

 the dish of sterilized sea-water from a distance of two or three 

 inches ; then water in the dish was taken up three times and 

 squirted at the surface. The control was simply transferred. 



An examination of these eggs was made at 10:15 P. M., when 

 their condition was not much different from those in Experiment I, 

 except that more showed effects of the agitation. The next morn- 

 ing, 10:15 A. M., the following results were obtained : 



Lot 

 Number. 



I control. 



2 



3 



4 



5 



6 



7 



8 



Experiment j. Eggs were transferred in the following lots 

 and squirted moderately as in Experiment 2. Thereafter they 

 were squirted again moderately at frequent (10-15 min.) inter- 

 vals, up to 4:40 P. M. Examined at 9 A. M. July 3 i. 



Lot Time Tr msferred Number Ciliated 



Number. from Beginning. in 2,000 Eggs. 



1 control. O min. o 



2 32 " 60 



3 60 " o 



4 i 20 " 4 



Experiment 4. The eggs were squirted violently, the water 

 vigorously agitated by squirting with a pipette, and then left 

 undisturbed. 



Condition at 8:45 A. M., July 31. Many fragments present. 



Lot Number. Time Transferred Number Ciliated 



from Beginning. in 2,000 Eggs. 



1 control. o min. o 



2 33 " 40 



3 60 " 2 



4 i 20 " o 



