Vol. XXX, February, 1916. No. 2 



BIOLOGICAL BULLETIN 



THE MITOCHONDRIA AND OTHER STRUCTURES 

 OBSERVED BY THE TISSUE CULTURE METHOD IN 

 THE MALE GERM CELLS OF CHORTHIPPUS CUR- 

 TIPENNIS SCUDD. 1 



MARGARET REED LEWIS, 



CARNEGIE INSTITUTION. 



WM. REES B. ROBERTSON, 



UNIVERSITY OF KANSAS. 



CONTENTS. 



Introduction 99 



Literature 100 



Living Material 100 



Fixed Preparations 101 



Method 101 



Observations 103 



General 103 



Vital Stains 104 



Nucleus 105 



Apical Cell 106 



Primary Spermatogonium 107 



Secondary Spermatogonium (Multiplication Stages) 107 



First Spermatocyte (Growth Stages) 108 



First Spermactocyte Division 108 



Second Spermatocyte (Interkinesis) 109 



Second Spermatocyte Division 109 



Spermatid and Spermatozoon no 



Discussion 112 



Conclusion 113 



INTRODUCTION. 



A study of the chromosomes of Chorthippus curtipennis by 

 Robertson (in press) led to the desire to study the mitochondria 

 and other structures of the cytoplasm in order to determine if 

 possible the bearing of the cytoplasmic structures upon the later 

 development. 



1 From the Marine Biological Laboratory, Woods Hole, Mass. 



99 



