FURTHER DEVELOPMENTS IN OVARIOTOMIZED FOWL. 289 



also were fully developed. When the bird moulted (1915) the 

 new feathers were those of the female throughout. As is fre- 

 quently the case, the bird moulted piece-meal, both kinds of 

 feathers co-existing at one time. Gradually, however, the male 

 feathers were replaced by feathers that could not be distinguished 

 from those of the female. When it is recalled that the Brown 

 Leghorns are practically identical in color with the Jungle fowl, 

 the change can readily be appreciated. The bird was not killed 

 as it was desired to keep her for further observation, but instead 

 she was opened on each side. Organs roughly similar to those 

 described for 1196 were noted. On the right side a strand of 

 tissue was traced posteriorly for a few millimeters but no strand 

 could be identified on the left. No trace of anything resembling 

 normal ovarian tissue was found. As the location of the incision 

 was unfavorable for finding the oviduct, this was not attempted. 

 A bit of each of the organs were removed and sectioned. The 

 histological findings were like those described below. 



A short time after the operation it was noted that the new 

 saddle feathers that came in were male-like in that they were 

 fairly long, and laced with bright yellow, though rather bluntly 

 pointed at the end and the central stipe though nearly black 

 was often sprinkled with brown to a greater degree than usual 

 for a male. The feathers of the tail coverts particularly con- 

 tained much brown. In the breast feathers, less change was 

 noticeable, most of the feathers remaining deep reddish salmon 

 though some of the feathers contained some black. Other 

 parts of the bird showed feathers much like those of the female 

 type. 



The third instance is that of a bird hatched June 22, 1913, and 

 castrated August 8, 1913. The juvenile female plumage was 

 well developed at the time. By early winter, however, this 

 plumage had given place to one nearly or quite identical with 

 that of the young male. The spurs developed fully but the 

 comb never became really male-like, but had rather the general 

 appearance of a Leghorn female's comb when straightened up. 

 This comb, however, never- loped but was always erect, an 

 exception not at all rare among Leghorn females. It was 

 anticipated that when the bird moulted during the summer 



