460 C. C. MACKLIN. 



through it from one side to the other of a membrane was not 

 found. Nuclear figures simulating this proved to be caused by 

 the close apposition of separate nuclear sacs, or by nucleoli, 

 mitochondria or folds of the nuclear membrane. 



The dyes, janus green and gentian violet, were toxic and their 

 presence in the cell was incompatible with its continued life. 

 They were, however, of service in quickly studying structural 

 details which were not discernible in the living state. 



Nuclear fragmentation, which differs in many ways from nu- 

 clear amitosis, is a pathological condition, and occurs in degener- 

 ating cultures. 



It is believed that the facts brought to light through the tissue 

 culture method may be applied to the interpretation of the 

 phenomena of normally developing cells. 



LITERATURE CITED. 



Child, C. M. 



'07 Studies on the Relation between Amitosis and Mitosis. IV. Nuclear 



Division in the Somatic Structures of the Proglottids of Moniezia. V. 



General Discussion and Conclusions Concerning Amitosis and Mitosis in 



Moniezia. BIOL. BULL., Woods Holl, XIII., 165-184. 

 'ii The Method of Cell-Division in Moniezia. BIOL. BULL., Woods Hole, 



XXI., 280-296. 



Churchman, J. W., and D. G. Russell. 



'14 The Effect of Gentian Violet on Protozoa and on Growing Adult Tissue. 

 Proc. Soc. Exper. Biol. and Med., N. Y., XL, 120-124. 



Isaacs, R. 



'15 A Mechanical Device to Simplify Drawing with the Microscope. Ana- 

 tomical Record, Phila., Vol. 9, 711-713. 



Karpow, W. 



'04 (Untersuchungen iiber direkte Zellteilung.) Inaug.-Diss. Moskau, 1904. 

 Refer, in Jahresber. ii. d. Fortschr. d. Anat. (Schwalbe), Jena, n.F.,X., 42. 



Lambert, R. A. 



'i2a Variations in the Character of Growth in Tissue Cultures. Anat. Rec. 



Phila., VI., 91-108. 

 'i2b The Production of Foreign Body Giant Cells in Vitro. Jour. Exper. 



Med., Lancaster, Pa., XV., 510-515. 



Lewis, M. R., and W. H. Lewis. 



"15 Mitochondria (and Other Cytoplasmic Structures) in Tissue Cultures. 



Amer. Jour. Anat., Phila., XVII., 339-401. 

 Maximow, A. 



'08 Ueber Amitose in den embryonalen Geweben bei Saugetieren. Anat. 

 Anz., Jena, XXXIII., 89-98. 



