28 J. E. WODSEDALEK. 



without division of the chromosomes. The latter phase of the 

 assumption is based on the fact that indications of the actual 

 division of the chromosomes in the primary spermatocytes are 

 extremely rare, and that occasionally it can be seen that the 

 nuclei actually enter the process of reorganization before the 

 chromosomes had divided. This is especially true in cells with 

 multipolar and double spindles. As to the nucleus outside of the 

 cell (Fig. 24), it may be suggested that the chromosomes con- 

 tained therein resembled in the metaphase stage of this cell the 

 group of chromosomes without spindle threads represented in 

 Fig. 32, and that later they were expelled from the cell after the 

 fashion shown in Fig. 23. The three chromosomes in the cyto- 

 plasm were probably treated with indifference by the three large 

 groups of chromosomes, being neither attracted nor repelled by 

 them, and thus remained scattered about in the cytoplasm. The 

 material necessary to construct a separate nuclear wall for the 

 three deserted chromosomes was apparently lacking. 



Another possibility as to the origin of the nuclei in question 

 is that the cell may have had two spindles, as are shown in Fig. 29, 

 with an additional group of chromosomes without the spindle 

 threads, for such cells were also observed. Still another possi- 

 bility is that the cell may have possessed three spindles like those 

 shown in Fig. 34 and that the chromosomes belonging to one of 

 the spindles were eventually expelled from the cell and there 

 reorganized a nucleus while the other two spindles remained 

 within the cell. 



There seems to be no doubt but that the chromatin material 

 found in the small nucleus on the outside of the cell shown in 

 Fig. 24, originally formed a part of the inner contents of the same, 

 for a distinct connection in the form of coarse granular threads 

 still persists between it and the chromosomes within the cyto- 

 plasm, as well as the two large nuclei themselves. The group of 

 sixteen chromosomes was no doubt expelled from the cell and 

 there formed a separate nucleus. The nuclear membrane is 

 thin but well defined. It can also be seen that a quantity of 

 cytoplasm was forced out with the chromosomes. Some of it, 

 very finely granular, persists around the nuclear membrane. 

 The rupture in the cell wall occasioned by the expulsion of such 



