FURTHER DEVELOPMENTS IN OVARIOTOMIZED FOWL. 2QI 



expected difficulties were met, so that it was deemed desirable 

 to proceed no further. 



There remain two other birds to be considered. Though 

 both were of hybrid origin their plumage was that of a typical 

 Brown Leghorn. At the time they were castrated, the female 

 juvenile plumage was well developed. After castration both 

 developed spurs and a perfect male coat of plumage but their 

 combs remained small. 



Number 4290 was killed November 25, 1914. The same sort 

 of organs on the site of the gonads were noted again. The other, 

 4471, is still alive and in perfect male plumage. In the summer of 

 1913, a female-like plumage developed, followed in the fall by 

 a return to the male plumage. In 1915 no change in plumage 

 color took place. When opened, October n, 1915, the same sort 

 of organs were found. Thus, in all but possibly one instance 

 there has been a development of glandular material on both sides. 

 The exception relates to the individual that was examined on 

 only one side. It seems probable that though numbers 3840 

 and 2087 were operated on just prior to their return to the male 

 condition the operation as such had nothing to do with the 

 results secured but rather that a change to this plumage would 

 have taken place as in the case of 4471. It is of course possible 

 that the operations accelerated a change about to take place. 

 It is quite possible, too, that the changes in plumage are cyclic 

 in nature, like the occurrence of the summer plumage in the 

 normal drake. 



Because it is desired to keep the birds alive for further observa- 

 tions, the structure of the organs cannot at present be given in 

 detail. It was thought at first that a small piece would suffice 

 for a determination of its structure, but the material thus far 

 examined indicates that the study will have to be made from the 

 standpoint of the organ as a whole. Owing to lack of material, 

 this cannot be attempted for some time to come. However, 

 the material on hand is sufficient to indicate something of its 

 nature. The following description is provisional: The histo- 

 logical findings vary from specimen to specimen and also in 

 different parts of tissue from the same bird. The differences, 

 however, are probably to be referred to developmental stages, 



