SEXUAL ELEMENTS OF THE GIANT SALAMANDER. 223 



No structures resembling spermatophores were discovered, and 

 there was nothing that would seem to give any indication of the 

 method by which the act of fertilization was accomplished. 



A single spermatozoon, as seen under a magnification of about 

 1,300 diameters, is shown in Fig. 3. 



Fairly good preparations were easily made by drying them 

 rapidly on the slide, and staining in haematoxylin and eosin. 



The nucleus, //, is very much elongated, so that it makes up 

 almost one third of the entire length of the spermatozoon. It is 

 capped, at its anterior end, by a sharp, gradually-tapering apical 

 body, a, which is plainly differentiated from the nucleus proper 

 by the fact that it does not take up the stain to any great ex- 

 tent. No structural details in the nucleus or apical body can be 

 discerned with the magnification used, nor is any middle-piece 

 distinguishable. The tail, which is comparatively stout, consists 

 of a central supporting fiber, s. f., which takes up the stain 

 slightly, surrounded by a transparent envelope, e, which does 

 not stain at all. The envelope is usually considerably wrinkled 



and twisted, probably by the rough method of fixation. 

 ZOOLOGICAL LABORATORY, 



SYRACUSE UNIVERSITY. 



