I4O R. W. GLASER. 



Spirillum from Periplaneta americana. 



Morphology. 1.5 per cent, neutral nutrient agar, short and 

 long rods. Some curved, others straight. In nutrient bouillon, 

 some straight rods and many curved. Average length 1.5-2^. 

 Stains readily. Gram negative. No endospores. Motile. A 

 single flagellum attached to one of the poles. 



Nutrient Agar Stroke. 1.5 per cent, neutral. Growth abun- 

 dant, filiform, flat, glistening, smooth, opaque, butyrus, odor ab- 

 sent, medium unchanged. 



Nutrient Bouillon. Ring, pellicle, clouding strong, odor ab- 

 sent, sediment abundant and flocculent. 



Gelatin Stab. Growth best at top, line of puncture filiform, 

 liquefaction begins in two days, craterif orm. Medium unchanged. 



Litmus Milk. No acid, coagulation prompt. Prompt re- 

 duction. 



Milk. Coagulation prompt, extrusion of whey. Consistency 

 unchanged. Medium slightly greened. No peptonization. 



Potato. Growth scanty, slightly spreading, flat, dull, smooth, 

 butyrous, odor absent, medium unchanged. 



Nutrient Agar Colonies. 1.5 per cent, neutral. Growth rapid 

 at 35 C., round, smooth, flat, edge entire, amorphous. Diam- 

 eter Y\-2. mm. 



Nutrient Gelatin Colonies. Growth slow, colonies minute. 

 Round, smooth, flat, edge entire, liquefaction saucer. 



Indol test negative. 



NH 3 test positive. 



Nitrates not reduced to nitrites. 



Diastase Test. Starch converted to sugar. 



Strong catalase reaction in bouillon cultures. 



No peroxidase reaction. 



FERMENTATION OF CARBOHYDRATES WITH FORMATION OF ACID AND GAS. 



Gas. Arid. 



Dextrose o o 



Levulose o o 



Saccharose o o 



Maltose o o 



Lactose o o 



Mannit o o 



