33O ROBERT H. BOWEN. 



Viewed on edge the bodies exhibit the characteristic " split " 

 (Text-figure ifr) a feature very possibly of wide occurrence 

 as it seems to be referred to in several published accounts of the 

 Golgi apparatus. Following the so-called chrome-osmium 

 methods much used by Gatenby, the appearance is quite different 

 (Text-figure ic). The peripheral rim stains only as a dark 

 crescent on one side of the disc of non-staining substance 

 (" archoplasm" of Gatenby) or occasionally the rim may stain 

 more completely, approaching the result obtained by impregna- 

 tion with osmic acid. By these methods I have, however, been 

 unable to demonstrate the " split " in an edgewise view (Text- 

 figure id). Without going further into the morphology of the 

 Golgi bodies, I would like especially to call attention to their 

 obviously duplex structure as demonstrated by the staining reac- 

 tions, a feature to which I shall return in a later section. 



In the final growth period of the large cells it is not uncom- 

 mon for the Golgi apparatus to undergo secondary changes. 

 Thus in Euschistus the bodies tend to fuse with each other, the 

 number in a single cell being reduced to ten or twelve. In Bro- 

 chymena this fusion is carried much further, and great, amor- 

 phous masses are formed, while in Nesara, on the other hand, 

 the individual bodies are very small. That these various morpho- 

 logic differences are of no particular importance is indicated by 

 the fact that in the subsequent divisions the general behavior is 

 the same in all three genera. 



The spermatocytes now enter upon the prophases, and until 

 the spermatids are finally formed the behavior of the Golgi 

 bodies is of great interest. The coincident series of events 

 through which the mitochondria and the chromosomes are pass- 

 ing should be borne continually in mind, for only by such a com- 

 posite picture can one form a proper conception of the mitotic 

 process as a whole. As in case of the mitochondria, it is the 

 small cell generations which offer the best material for the gen- 

 eral study of the Golgi bodies during the prophase and division 

 stages, and I will accordingly refer to the large cells only by way 

 of comparison. 



The early prophase probably marks the beginning of the steps 

 preparatory to the division of the Golgi apparatus, but it is only 



