286 



E. E. JUST. 



June 17, 8:00 A.M. Eggs from dishes show cytolysis as 

 follows : 



This observation on washed eggs shows very clearly that over- 

 exposed eggs thirteen hours after treatment are cytolyzed to a 

 greater extent than under-exposed. The control eggs cytolyze 

 more slowly than the under-exposed eggs which is an exception 

 to the rule. 



Experiment 8 B. June 17. Eggs from a very good female ex- 

 posed to butyric acid sea-water from 15 to 120 seconds. Control 

 in normal sea-water show some eggs devoid of jelly. Four hours 

 later these eggs show per cent, cytolyzing as follows : 



No. 



Four hours after butyric acid treatment we find over-exposed 

 eggs 100 per cent, cytolyzed. In most cases this percentage is 

 reached after three hours. Cytolysis begins in these eggs within 

 an hour after treatment when most of the other eggs including 

 the control are intact. The beneficial effects of short exposure 

 to butyric acid are strikingly brought out in this observation. 



Experiment 9 B. June 19, 7:00 P.M. Eggs treated for o, 15, 

 20, 25, 35, 40, 60, 90, and 120 seconds. 



June 20, 8:00 A.M. These eggs after thirteen hours show 

 the following: 



No. 



