STUDIES ON INSECT SPERMATOGENESIS. 327 



that in preparation for and during the maturation divisions the 

 threads are under the visible " control " of the centrioles, (4) 

 and that by this means each spermatid receives an accurate quar- 

 ter of the mitochondria contained in the first spermatocyte. 



One important cytoplasmic structure remains to be considered, 

 viz., the Golgi apparatus, a cell element whose role in spermato- 

 genesis has thus far been largely mistaken or overlooked, but 

 which, as I hope to show, must play a significant part in all 

 future analysis of the sperm. The term " apparatus," in its 

 original meaning, is rather a misnomer in the Hemiptera, for in 

 these forms this structure is generally composed of many separate 

 Golgi bodies (Fig. /). To one long accustomed to the appear- 

 ance of Flemming preparations of insect germ cells, the Golgi 

 preparations are a revelation ; for the mitochondria and chromo- 

 somes fade into a shadowy background upon which the Golgi 

 bodies appear in brilliant black. Indeed a successful prepara- 

 tion is so nearly diagrammatic that the main outlines can be made 

 out almost at a glance. For the purposes of this report, I have 

 abridged the description of the Golgi bodies, attention being cen- 

 tered principally on their behavior in the maturation divisions. 

 This account also is based on Euschistus. 



For a starting point we may, as in the case of the mitochon- 

 dria, take the spermatogonia in the " rosette " stage. In these 

 early generations of spermatogonia the Golgi apparatus consists 

 of a few scattered bodies which show no particular orientation 

 with respect to any other part of the cell. In later generations 

 the number seems to be slightly increased and a tendency for 

 the bodies to collect in the neighborhood of the mitochondrial 

 " cap " is apparent. Finally in the last spermatogonial genera- 

 tion (or possibly stage a of Wilson), this orientation is completed 

 (Fig. i), and the Golgi bodies are definitely restricted to that 

 portion of the cell in which the mitochondria are aggregated. In 

 dividing spermatogonia the Golgi bodies are broken up into 

 small granules which seem to have no definite method of dis- 

 tribution to the daughter cells. 



The synaptic stages offer nothing of interest but, as the mito- 

 chondria spread out in the diplotene, the Golgi apparatus likewise 

 enters upon a period of growth and activity. As in the case of 



