CHROMOSOMAL VESICLES IN I'll ASEOLUS. 2IJ 



interpretations were entirely beside the mark and his paper did 

 not attract widespread attention. 



This author figures the linin strands in resting nuclei as well as 

 their development in the telophase. His observations on both 

 of these phases have been violently attacked by Martins Mano 

 (1905). Immediately after the appearance of Wager's article 

 Martins Mano undertook to repeat his work on Phaseolus. 

 The latter concluded that the nuclear reticulum arises by the 

 "branching and anastamosing " of telophase chromosomes and 

 that the nucleolus appears independently and rather suddenly 

 after the reconstruction of the daughter nuclei is almost complete. 

 His figures of the telophase, which led to the above incorrect 

 interpretations, can be explained on the basis of Fig. 7. This 

 figure illustrates a cell through which the plane of the section 

 passed in such a way that a very small portion of one of the 

 daughter nuclei appears in the section. The non-vacuolized 

 portions of the chromosomes are entirely absent and, since only 

 a small part of the side of the reconstituting nucleus is present the 

 proximal ends of the chromosomal vesicles appear in cross section 

 and consequently fail to show the even division of the nucleus by 

 parallel linin strands. The other nucleus in this dividing cell 

 exhibits the normal picture. Martins Mano admitted that 

 structures similar to Wager's description were found, but claimed 

 that the ones which he figures were of more frequent occurrence. 

 In the light of the present work it can be safely said that Martins 

 Mano must have exercised a great deal of patience in searching 

 for the abnormal and infrequently occurring cells which he figured 

 and with which he tried to support the theory that the recon- 

 struction of nuclei is by the branching and anastamosing of telo- 

 phase chromosomes, a theory which is incorrect in the case of 

 Phaseolus. Martins Mano says that the linin strands which 

 Wager figures as running from the nucleolus to the nuclear 

 membrane do not exist, but are a part of the reticulum above the 

 nucleolus. Their presence in the dormant seed, when the 

 reticulum disappears shows that \Vager was correct. 



The great majority of described cases of chromosomal vesicles 

 appearing during the reconstruction of nuclei are from animal 

 eggs. Such structures have been described and discussed so 



