35O WOOLFORD B. BAKER. 



chromosomes, as soon as formed, become quite tortuous in the 

 limited space. The enlarging endosome with the budding off of 

 the kinetic complex tends to force the chromosomes toward the 

 periphery of the nucleus thus forming many twists and loops. 

 When these chromosomes come to lie radially around the 

 elongated endosome, preceding the longitudinal splitting, they 

 appear as loops whose arms are rather closely pressed together. 

 Thus at one focus one can distinguish a number of pairs of 

 distinct chromosomes, but as the focus is changed the ends may 

 be found to be connected into the loop. The longitudinal split 

 begins at the end next to the endosome and proceeds toward the 

 periphery. Thus, if an apex is inward, as is frequently the case, 

 a double V-shaped figure is produced as the split halves separate. 

 An X shape or a figure 8 shape is produced if the free ends are 

 turned inward. In either case, the pulling apart of the split 

 halves forms the equatorial plate figure, or as it might be better 

 expressed, the "equatorial cylinder." 



Anaphase. From the equatorial cylinder stage the ends of the 

 split chromosomes pull apart and an appearance of a transverse 

 splitting of chromosomes is the result. This perhaps accounts 

 for some of the earlier descriptions and widely quoted figures 

 which show a transvers splitting of chromosomes in Euglena. 



In the late anaphase, the chromosomes begin to show evidence 

 of a return to the typical vegetative condition. Dehorne (1920) 

 describes them as going back into the continuous spireme stage of 

 the resting nucleus. It is true that the exact identity of the 

 chromosomes tends to be lost as they begin to condense into the 

 granular condition, but little evidence is found that a continuous 

 spireme is constructed. 



Telophase. Careful search was made for a precocious splitting 

 at this stage in order to determine whether the description as 

 given by Tschenzoff for Euglena viridis held also for Euglena 

 agilis. Aside from the apparently diffuse nature of the chromo- 

 somes and their change in intensity of staining reaction, no 

 definite evidence of a split was observed. 



The Residual Mass from the Kinetic Complex. 



Blochmann (1894) found in Euglena velata a body in the 

 cytoplasm, often lying near the nucleus but sometimes far from it. 



