364 JOSEPH HALL BODINE. 



cutting off the tip of the feet, puncturing the animal by means of 

 needles, insertion of electrode vessel directly into the body cavity, 

 etc. No marked differences in pH of the blood obtained by these 

 different methods was noted. The method found most satis- 



TABLE i. 



SPECIES: Chorlophaga viridifasciata. 

 Age, Color, Sex. pH of Blood- 



nymph brown cT 6.83 



nymph green 9 6.83 



nymph brown $ 6.84 



nymph brown 9 6.86 



adult cf 6.62 



adult cf 6.43 



adult cf 6.67 



adult o* 7.05 



adult d" 1 6.85 



adult 9 6.65 



adult 9 6.94 



adult 9 6.51 



adult 9 6.95 



adult 9 6.73 



adult 9 6.93 



Average 6.79 



Range 6.43-7.05 



Explanation of Table I. 



Data taken at random from many individuals showing pH of blood of Chorto- 

 pkaga viridifasciata. Animals all taken from out-of-doors. Some bloods tested at 

 once. Other animals kept in laboratory and fed lettuce over different periods. 

 Samples of blood tested regularly. Average given for all individuals tested. 



factory and least harmful to the grasshopper, however, was a 

 small needle puncture into the mid-lateral abdominal wall. Only 

 a very small amount of blood is thus lost by the insect and the 

 wound is quickly sealed. Sufficient blood (approximately o.oi 

 cc.), however, is obtained in this way for electrometric determi- 

 nations of pH values. The grasshopper was usually punctured 

 and the blood immediately drawn up into the electrode vessel 

 with practically no exposure to air. As a matter of fact, identical 

 results were obtained with blood taken from the interior of the 

 animal by the insertion of the electrode vessel directly into the 

 animal as by the method of puncturing and then immediately 



