ELIZABETH KINNEY. 



(b) Observation. In the gland of //. cunea after fixation by 

 the Regaud method, the presence of a clear, hyalin layer of 

 material between lumen and cell-wall is found to be quite 

 generally present in both the middle and anterior regions. 

 (Figs. 7, 8, 9, 12.) This layer is always found to be very closely 

 connected with the cell-wall, and yet to be perfectly distinct 

 from the protoplasm. Oftentimes large vacuoles may be seen in 

 the structure (Fig. 7), but these are always on the edge toward 

 the cell and may be explained as the result of shrinkage at the 

 time of fixation. This vacuolated condition occurs with greater 

 frequency in the posterior portion of the middle region where 

 the layer is thicker and less regular in form. 



Posteriorly, in the secreting region, no indication of an intima 

 is found except for the presence of a thin membrane along the 

 inner edge of the cells. The cells usually have an irregular 

 surface on the side toward the lumen, the protoplasm seeming 

 to be in very close connection with the cavity (Fig. 2), but after 

 fixation with Champy, Gatenby, or Benda preparations, this 

 membrane is quite definite (Fig. i). 



Toward the more anterior portion of the middle region, fibrillae 

 may be seen extending through the clear hyalin substance and 

 around the lumen. In longitudinal sections through the edge of 

 the lumen, these structures appear to extend from the edge of 

 the cell on one side of the lumen to the cell on the other side 

 (Fig. 10), and to take their origin along the margin of the proto- 

 plasm. These fibrilhe stain darkly with iron haematoxylin and 

 dark red with Altmann's acid fuchsin after fixation by the 

 Champy method. In general, the fibrilhc tend to be arranged 

 in parallel series and some evidence has been found of slight 

 anastomosis between fibril he in the more posterior region. After 

 the tube has become narrowed to form the conducting portion, 

 fibrillae disappear leaving the intima clear and structureless. 



(c) Critical. The fact that the intima is present in the 

 anterior and middle portions of the gland as a thickened layer 

 -eems to indicate that this structure is not connected with active 

 secretion but must serve some function related to conduction of 

 secreted products to the outside. A copious supply of secretion 

 i- being poured continually into the reservoir from the posterior 



