RELATIONSHIP HKTWEEN A RED TORULA AND A MOLD. 157 



In a fluid synthetic medium the reaction at which good growth 

 ceases was found to be about Ph 2.8, while that at which even 

 sparse growth ceases was found to be about Ph 2.0. 



Pigment formation seems to be slightly favored by the more 

 acid medium. 



The organism will grow on all of the ordinary media, with good 

 pigment production. 



Dextrose is fermented but slightly, even after 20 days growth. 

 Acid is formed, but no alcohol could be detected by the method 

 of distillation. No gas is formed. Lactose, saccarose, mannite 

 and xylose are not fermented when used in extract bouillon. 



Growth in a suitable synthetic medium of about Ph 3.2 l 

 occurs after five days as a dirty looking sediment. After standing 

 for twenty-one days or more, however, heavy growth of coral 

 pink sediment gradually appears, and, if left undisturbed, a 

 thin pink scum forms, and a ring appears at the junction of the 

 fluid surface and the vessel wall. 



The torula is an ae'robe. When an inoculated infusion agar 

 slant was placed in a Brown (6) anaerobic jar, no growth occurred 

 after ten days at room temperature. Upon removal of this 

 slant from the jar, good growth occurred after forty-eight hours. 

 A flask of the synthetic medium described above, which had 

 been heated for some time in order to invert some of the sucrose, 

 was also placed, after inoculation, in an anaerobic jar for one 

 week. No growth occurred. Growth appeared quite promptly 

 after removal of the flask from the jar. 



Morphologically, the organism is ellipsoidal, the cells being 

 from 5 to 7 ju long by about 3 to 5 ^ wide. No spores have been 

 observed. Typical budding occurs. 



A study of the pigment showed it to belong to the class of 

 acid-alkali indicators. The growth on an agar slant was treated 

 with nil acid. No change in color took place. On treatment 

 of the same growth with n/i alkali however, the color turned to a 

 brown-orange and the red color could be restored by further treat- 

 ment with the acid. The change in color was not striking, but 

 definite. 



1 This medium is simply solution (A) of the agar described above, but containing 

 1,000 cc. of water instead of 500. 



