402 



C. E. GATES. 



This endosome has either one large, or several smaller, trans- 

 parent, vacuole-like areas. The nuclei of dead parasites are 

 always found in the unhranched portion. 



O 



o 

 C 



A B 



FIG. 3. A. Nucleus from trophozoite 1.3 mm. in length. (Camera lucida.) 

 X ca. 335. e., endosome; n.m., nuclear membrane. B. Nucleus from larger 

 trophozoite. (Camera lucida.) X ca. 350. C. Paraglycogen granules. (Camera 

 lucida.) X ca. 350. 



Live attached parasites have not been studied but in living 

 detached forms two series of movements are noticeable. The 

 first series may be described as waves of peristaltic contraction 

 passing along the trunk and rami of the first order. These 

 peristaltic contractions produce a back and forth churning of 

 the endoplasmic contents in the trunk, from the trunk into the 

 primary rami, and back and forth in the rami. By these con- 

 tractions the nucleus is squeezed to and fro from one end of the 

 trunk to the other. In two of the living specimens examined the 

 nucleus remained in the unbranched portion of the parasite 

 during the whole period of observation. In the other animal the 

 nucleus frequently passed into one or the other of the primary 

 rami. In the primary ramus the nucleus was either rolled back 

 and forth several times or at once passed back into the trunk. 

 The nucleus moved with equal ease and frequency into either 

 one of the primary rami but never passed into the secondary 

 rami which were narrower than the nucleus. It should be noted 

 that in this third animal the ramification began unusually near 

 the blunt end so that the trunk portion was less than one third 

 of the total length of the parasite. 



The second series of movements may be called rotation move- 

 ments and result in a spiral twisting of the primary rami and 

 their branches back and forth on their own axes. 



