414 ELIZABETH KIXNEY. 



as the nuclear bodies drew the conclusion that the cytoplasmic 

 granules are derived from the nuclear bodies (nucleoli) by 

 migration of the latter. Tanaka ('u) believes the fibroin, or 

 silk core, to be a product of the gland cell substance, visible only 

 when the secretion has accumulated to give rise to fine fibroin 

 droplets which unite to form larger droplets. Yamanouchi ('22) 

 likewise believes that secretion is produced in the cell. 



Whether or not these cytoplasmic bodies are produced in the 

 cell or in the nucleus, it seems to be generally concluded that they 

 are directly connected with the products of secretion. 



From observation of cytoplasmic granules in the cells of 

 //. cunea, it was found that they average between i micron and 

 2 micra in diameter, or approximate the size of the nuclear 

 bodies. The cytoplasmic granules tend to increase in size as 

 they approach the lumen and finally, the droplets of secretion 

 when passed into the cavity coalesce and become of considerable 

 size (Fig. 16). Evidence of active secretion is correlated with an 

 abundance of cytoplasmic bodies present in the cell, but not, as 

 might be expected, with a decrease in the number of nuclear 

 bodies present. It seems, therefore, that if the nucleus does 

 play an active part in the formation of secretion that there is 

 always an excess of nuclear bodies stored in the branched nucleus, 

 and while the drain upon one part may be great, the reserve 

 from another part of the nucleus may supply the demand. 



The cytoplasmic bodies are found in all parts of the cell, but 

 usually most abundantly between portions of a nucleus or 

 between the nucleus and the lumen (Fig. i). These bodies 

 usually react to acid stains, but are generally stained black with 

 iron hsematoxylin. (See chart.) 



That no cytoplasmic bodies are present in the middle section 

 is difficult to determine with absolute certainty. If the nucleus 

 with its nucleoloid bodies and nuclear bodies is responsible for 

 secretion, surely secretion should occur in this region, for both 

 types of bodies are present in the nuclei. Material fixed by the 

 Gatenby method and stained with iron ha>matoxylin and acid 

 fuchsin furnished the only indication that cytoplasmic bodies 

 may be present in the reservoir region. The bodies took a light 

 pink stain very similar to that of the clear, structureless substance 



